Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Acetaminophen Overdose Reveals PAR4 as a Low Expressing but Potent Receptor on the Hepatic Endothelium in Mice Arteriosclerosis, Thrombosis, and Vascular Biology Oxidative stress mediates end organ damage in a novel model of acetaminophen toxicity in Drosophila Scientific Reports Acetylcysteine for Acetaminophen Poisoning New England Journal of Medicine Full article: The development and hepatotoxicity of acetaminophen: reviewing over a century of progress